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Clean technology company BioLargo Inc. (BLGO:OTCQX) announced compelling new findings that its proprietary Aqueous Electrostatic Concentration (AEC) "forever chemicals" solution achieves over 99% removal of ultrashort-chain per- and polyfluoroalkyl substances (PFAS), including trifluoroacetic acid (TFA).

TFA is a notoriously persistent and mobile contaminant, easily moving through the environment due to its resistance to typical environmental breakdown, as it contains only two fluorinated carbon atoms (C2). Commonly found in industrial solvents and reagents, TFA is a byproduct of many industrial processes.

A study in Environmental Science and Technology revealed that TFA accounted for more than 90% of the total PFAS mass (of 46 individual PFAS analyzed) in various drinking water sources in Germany. This breakthrough represents a significant advancement in combating PFAS pollution, especially for compounds like TFA that have evaded conventional treatment methods due to their small molecular size and high solubility.

"Ultrashort-chain PFAS are among the most challenging to capture and remove," said BioLargo Water President Randall Moore. "Our AEC technology has now demonstrated the ability to concentrate and eliminate these compounds with unprecedented efficiency. This further positions BioLargo as a leader in the current and next-generation PFAS treatment industry."

BioLargo is made up of subsidiaries that work in different sectors, a "family of companies," including ONM Environmental, BioLargo Engineering, BioLargo Water, BioLargo Energy Technologies, Clyra Medical Technologies, and the new BioLargo Equipment Solutions & Technologies Inc. (BEST) subsidiary.

'A Game-Changer'

The company's internal testing, conducted alongside third-party laboratories, confirms that AEC consistently removes TFA and other ultrashort-chain PFAS at rates exceeding 99%, without producing harmful byproducts or secondary waste streams, BioLargo said. These results build on BioLargo's previously validated performance with long-chain PFAS such as PFOA and PFOS.

The BioLargo solution facilitates the efficient removal and concentrated collection of PFAS, followed by a proprietary destruction technique. BioLargo's AEC system employs electrostatic forces to selectively attract and isolate charged contaminants from water, enabling scalable, energy-efficient treatment across a wide range of PFAS profiles. The technology is currently being deployed commercially in North America.

"This is a game-changer for communities and any industry grappling with PFAS contamination," said BioLargo Chief Executive Officer Dennis P. Calvert. "We now have solid evidence in hand that our technology can tackle the full spectrum of PFAS compounds — including the ultrashort-chain molecules that others leave behind. We are actively engaged in discussions with potential partners and customers to begin ramping up our commercial activities."

BioLargo has crafted a comprehensive "full-service" water treatment solution that integrates AEC technology with engineering design, installation, PFAS destruction, field testing, and maintenance capabilities, providing clients with a genuine turnkey solution that can generate early revenue for the company in a project's lifecycle.

"While it has taken a long time for BioLargo to achieve success in the water treatment industry, our work has resulted in the company achieving recognition for its role in technology innovation and thought leadership, culminating in our PFAS technology leaders routinely being engaged as expert speakers and trainers for the industry, as well as the appointment of BioLargo's CEO to the U.S. Department of Commerce International Trade Administration's Environmental Technologies Trade Advisory Committee (ETTAC) and as Chairman of its Enabling Innovative Technologies (EIT) subcommittee," Calvert wrote.

For instance, the company exhibited with a booth at WEFTEC 2025, the world's largest water quality event in North America, in Chicago from September 29 to October 1.

PFAS Solution 'Even More Robust'

Chris Temple of The National Investor noted in a September 29 update that the company had revoked the technology licensed to the Pooph! Line of pet odor formulations to the company manufacturing and distributing the popular product for owing nearly US$4 million to BLGO.

Pooph's rise to national prominence as a consumer brand was facilitated by BioLargo's products, technical and commercial expertise, extensive market experience, and the collaborative partnership between the parties, the company noted. Driven by BioLargo's proprietary technology, Pooph products achieved over US$50 million in annual sales and garnered tens of thousands of positive customer reviews.

Looking ahead, BioLargo retains the option to "rebrand" its formulations, as the Pooph! name belongs to Ikigai Holdings, meaning it would be marketed under a new identity.

The loss of this revenue stream has certainly been a setback, Temple said, but financially the company remains stable, partly due to financings that have supported other subsidiaries moving toward their own larger anticipated revenues, particularly Clyra Medical in the near term and BioLargo's PFAS solutions.

"Just this morning, BioLargo announced that its regimen to remove PFAS 'forever chemicals' from water is even more robust," Temple said of the company's latest news release.

The newsletter writer also said he expects to visit the energy division in Oak Ridge, Tennessee, where the company is working on its new battery technology, in a couple of weeks.

The Catalyst: Stricter Federal Regulations

The Trump administration has cut nearly US$15 million in funding for research into PFAS contamination on U.S. farmland, halting studies that health advocates deem crucial for understanding a major source of food contamination, Tom Perkins reported for The Guardian on July 15.

Recent findings have shown that PFAS-laden pesticides and sewage sludge used as fertilizers can contaminate soil, with these chemicals subsequently transferring to crops and nearby water sources. The impact on individuals consuming products from these farms remains uncertain, the author noted.

"We have to do this research and take steps to not just make sure that our food supply is safe, but also ensure our farms and farmers are safe," said Bill Pluecker, a Maine state representative and public policy organizer at the Maine Organic Farmers and Gardeners Association, which has advocated for stricter sludge regulations, according to Perkins.

PFAS includes a group of approximately 15,000 compounds that persist in the environment in the environment and human body and are linked to various severe health issues, including cancer, liver disease, kidney problems, high cholesterol, birth defects, and weakened immunity.

Stricter federal regulations on PFAS are likely to prompt entities such as manufacturers, municipalities, and utilities to manage liability risks more proactively, according to a report by Bloomberg Professional Services in June 2024. "Complying with the rules and cleaning up contamination could require US$300 billion of additional spending by 2040, nearly US$200 billion of which may flow to environmental companies," the report said.

Ownership and Share Structure

About 13.8% of BioLargo is owned by insiders and management, according to Refinitiv. They include Chief Science Officer Kenneth Code with 8.19%, CEO Calvert with 3.25%, and Director Jack Strommen with 1.58%.

About 0.04% is held by the institution First American Trust, Refinitiv said.

The rest, about 85%, is retail.

Its market cap is US$52.35 million, with about 308.99 million shares outstanding and about 266.36 million free-floating. It trades in a 52-week range of US$0.32 and US$0.14.